American Foundation for Donation & Transplantation
Molecular Typing 2000 Manual
The first edition of this manual, published in 1994, provided guidance in the strange, new world of HLA typing with DNA, primers, and probes. The field has moved very rapidly since 1994, and now it is time for a new edition which reflects the progress that has been made and gives direction for the future.
The first section of the 2000 edition addresses the concepts that undergird molecular methods. The second section is procedure-oriented but emphasizes the "why" of the methodology rather than the "how to". Remaining sections include recommendations about how to set up a DNA laboratory, quality assurance and quality control issues, suggestions about the way to approach an NMDP donor search, helpful and relevant web sites, and the current ASHI standards.
CONTENTS
INTRODUCTION TO CONCEPTS
Basic Molecular Biology
Immunobiology of HLA
Comparison of Molecular and Serological HLA Typing-Editorial
HLA and Transplantation
Allogeneic Marrow Transplantation
Solid Organ Transplantation
Polymerase Chain Reaction as the Basis for Detection of HLA Genes
PROCEDURES FOR DNA TYPING
Preparation of Material for Molecular Typing
Molecular Typing Using Sequence-Specific Primers
Laboratory Protocols for In-House PCR-SSP
Commercial Products for HLA Typing by PCR-SSP
Molecular Typing Using Sequence-Specific Oligonucleotide Probes
HLA Class I Molecular Typing by Sequence Specific Oligonucleotide Probes
Reverse Dot Blot DNA Typing Technique for HLA-DRB and DQB
HLA-DRB Typing Using an In-House ELISA-Based Oligoprobe Assay
Sequence-Based Typing
Trouble Shooting
LABORATORY DESIGN AND METHOD SELECTION
QUALITY ASSURANCE IN THE DNA LABORATORY
SEARCHING FOR A MARROW DONOR THROUGH THE NMDP: SUGGESTIONS FOR DIRECTING A SEARCH
INTERNET TIPS
Helpful Web Sites
Downloading Sequence Data from the Internet
ASHI STANDARDS, 1998
To order a copy call AFDT or mail a copy of the order form and a check to:
8154 Forest Hill Ave, Suite 3
Richmond, VA 23235
Phone: (804) 323-9890
Toll Free: (800) 543-6399
FAX: (804) 323-1300
